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Table showing sequence of primers for Real Time PCR
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Table showing sequence of primers for Real Time PCR
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Image Search Results


Table showing sequence of primers for Real Time PCR

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Table showing sequence of primers for Real Time PCR

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: Sequencing

Level of expression of (a): BRCA2 , (b): XPD and (c): APE1 genes in the tumour tissue, blood and NAT of HNSCC patients, respectively, normalised with level of respective genes in age- and gender-matched control blood samples. AU stands for arbitrary units. Difference of gene expression among the stages was evaluated by one way ANOVA with Tukey’s post-hoc test and was not found to be statistically significant.

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Level of expression of (a): BRCA2 , (b): XPD and (c): APE1 genes in the tumour tissue, blood and NAT of HNSCC patients, respectively, normalised with level of respective genes in age- and gender-matched control blood samples. AU stands for arbitrary units. Difference of gene expression among the stages was evaluated by one way ANOVA with Tukey’s post-hoc test and was not found to be statistically significant.

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: Expressing

Alterations in the level of BRCA2, XPD and APE1 proteins determined by (a): western blotting technique, with β-actin protein serving as loading control; and slot blotting followed by immunoprobing for (b): BRCA2, (c): XPD and (d): APE1 proteins in the PBLs of HNSCC patients compared to age- and gender-matched controls, with respective Ponceau S stained slot-blots serving as loading control. ** denotes statistical difference between controls and cases at p < 0.01; *** denotes statistical difference between controls and cases at p < 0.001. AU stands for arbitrary units.

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Alterations in the level of BRCA2, XPD and APE1 proteins determined by (a): western blotting technique, with β-actin protein serving as loading control; and slot blotting followed by immunoprobing for (b): BRCA2, (c): XPD and (d): APE1 proteins in the PBLs of HNSCC patients compared to age- and gender-matched controls, with respective Ponceau S stained slot-blots serving as loading control. ** denotes statistical difference between controls and cases at p < 0.01; *** denotes statistical difference between controls and cases at p < 0.001. AU stands for arbitrary units.

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: Western Blot, Staining

Representative slot-blots showing (a): altered expression of BRCA2, XPD and APE1 proteins in the PBLs of HNSCC patients in comparison to those of age- and gender-matched controls. (b): The corresponding Ponceau S stained slot-blots to verify equal loading of samples.

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Representative slot-blots showing (a): altered expression of BRCA2, XPD and APE1 proteins in the PBLs of HNSCC patients in comparison to those of age- and gender-matched controls. (b): The corresponding Ponceau S stained slot-blots to verify equal loading of samples.

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: Expressing, Staining

Comparative analysis of the expression profile of BRCA2, XPD and APE1 proteins in the PBLs of individual HNSCC patients normalised with respect to the corresponding levels in the PBLs of age- and gender-matched controls. Each row denotes individual patients containing patient ID and each column represents the fold level of the designated protein. The highlighted patients IDs are the 12 HNSCC patients from whom tissue biopsy, blood and adjacent normal tissue were obtained for real-time analysis study.

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Comparative analysis of the expression profile of BRCA2, XPD and APE1 proteins in the PBLs of individual HNSCC patients normalised with respect to the corresponding levels in the PBLs of age- and gender-matched controls. Each row denotes individual patients containing patient ID and each column represents the fold level of the designated protein. The highlighted patients IDs are the 12 HNSCC patients from whom tissue biopsy, blood and adjacent normal tissue were obtained for real-time analysis study.

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: Expressing

Correlation between levels of BRCA2, XPD and  APE1  proteins and different stages (I, II, III, IV) of HNSCC.

Journal: ecancermedicalscience

Article Title: Low level of BRCA2 in peripheral blood lymphocytes is associated with an increased risk for head and neck squamous cell carcinoma (HNSCC) in a population of North-East India: a case-control study

doi: 10.3332/ecancer.2023.1503

Figure Lengend Snippet: Correlation between levels of BRCA2, XPD and APE1 proteins and different stages (I, II, III, IV) of HNSCC.

Article Snippet: Primary antibodies anti-BRCA2 (ab27976), anti-XPD (ab167418), anti-APE1 (ab137708) and Prism ultra protein ladder 10–245 kDa (ab116028) were obtained from Abcam, UK.

Techniques: